TurboKnockout® gene targeting service is dependant on traditional embryonic stem cell (ESC)-mediated targeting techniques, which is often employed for complex gene modeling projects to supply C57BL/6 or BALB/c mouse models with accurate genetic modification and no off-target effects. Cyagen knock in mouse model Our TurboKnockout® service can offer you conditional knockout, reporter knockin and humanization mouse models as fast as 6-8 months.
Cyagen's TurboKnockout® Gene Targeting Service
This proprietary gene targeting method is enabled by two innovations:
(1) An excellent competent ES cell line that generates 100% ESC-derived founder mice as opposed to chimeras, eliminating the necessity to screen for germline transmission.
(2) A selection cassette on the targeting construct effective at removing itself after ES cell targeting without the need to breed to Flp deleter mice.
These innovations eliminate two generations of breeding, shortening production time by 4-6 months when compared with industry standard. For conditional knockouts, it could be possible to breed founders right to your tissue-specific Cre mice.
Advantages of TurboKnockout® Gene Targeting
Compared with CRISPR/Cas9-based techniques, TurboKnockout® is free of patent disputes, provides precise models on a similar timeline, and may be the technology of choice among our clients performing drug development projects.
Freedom to Operate: Technology of choice for drug development projects
Success Rate: 100% guaranteed germline transmission (GLT)
Turnaround Time: Founders as fast as 6-8 months
Large Fragment Knockin: Insert fragments up to 300 kb
Flexible Floxing: No size limitations for your floxed region
100% Money-Back Guarantee
